Fold induction and function of the 10 most upregulated genes in macrophages co-cultured with immune serum in combination with larvae as compared to larvae alone.

Bone marrow derived macrophages (106/ml) were cultured with larvae (500/ml) in the presence or absence of immune serum. Cells were detached from plates or larvae and passed through 40 µm filters before collection for RNA extraction. Changes in gene expression were identified by whole mouse genome microarray (Affymetrix) and confirmed by qPCR (for genes in bold). For a description of the microarray analysis, see Text S1.