TRANSGENIC METHOD OF OBTAINING BLUE FLOWERS BY CATALYZING GLUTAMINE TO SYNTHESIZE INDIGO
收藏国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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资源简介：
A transgenic method of obtaining blue flowers by catalyzing glutamine to synthesize indigo is provided. The steps thereof comprise: 1) respectively cloning a Sfp gene encoding phosphopantetheinyl transferase and a bpsA gene encoding indigo synthase downstream of a plant promoter in a plant-promoter-containing plasmid; 2) amplifying the obtained plasmid in E. coli and then transferring the same to Agrobacterium tumefaciens; and 3) transferring DNA containing Sfp and bpsA into a plant. The blue flowers produced by the present invention have various characteristics of natural flowers， being fresh， flower-scented， non-color-fading， and non-toxic. The transgene-encoded enzyme and the produced indigo are not in the vacuole and are not affected by the low pH of the plant vacuole， thereby resulting in a pure blue color. The precursor of the blue matter， i.e.， the substrate of the enzyme， is glutamine， which is abundant in plants. The enzyme catalysis reaction comprises a single step， and the transgenic transformation can be carried out on natural white flowers.
提供机构：
国家林业和草原科学数据中心
创建时间：
2023-02-12
