Data Sheet 1_Chemokine profile in the serum of patients with leptospirosis.xlsx

IntroductionLeptospirosis is a global zoonosis that affects more than one million people per year, with a lethality rate of approximately 15%. Chemokines are crucial in the immune response against Leptospira, recruiting leukocytes to the site of infection and regulating immune activity. In previous studies, we have shown that CCL2, CXCL5, and CCL8 are involved in the leptospirosis process, although the mechanisms are not understood. MethodsIn this study, we present the frequency of Leptospira serovars in human samples. We then evaluated the profile of various chemokines in sera from patients diagnosed with leptospirosis, assessing the possible correlation between them. Moreover, we evaluated the changes in the chemokine profile on different days after the first symptoms. The frequency of the Leptospira serovars in human samples is presented. Results and discussionThe main findings were that CCL5, CXCL5, and CXCL9 are highly expressed during leptospirosis, indicating a special role of these molecules in the immunity and pathogenesis of the disease. The correlation analysis of detected chemokines CXCL11, CXCL9, CCL3, and CCL2 helps to clarify the role of each cytokine in leptospirosis. The possible use of CCL5 as a biomarker for complementary diagnosis of the disease is suggested.

引言钩端螺旋体病是一种全球性的动物源性人畜共患病，每年影响超过一百万人口，其致死率约为15%。趋化因子在抵抗钩端螺旋体的免疫反应中起着至关重要的作用，它们能够募集白细胞至感染部位并调节免疫活性。在先前的研究中，我们已证实CCL2、CXCL5和CCL8参与钩端螺旋体病的发病过程，尽管其作用机制尚不明确。方法在本研究中，我们展示了人类样本中钩端螺旋体血清型的频率。随后，我们对被诊断为钩端螺旋体病的患者的血清中各种趋化因子的特征进行了评估，以探讨它们之间的可能相关性。此外，我们还评估了不同时间点趋化因子特征的改变。展示了人类样本中钩端螺旋体血清型的频率。结果与讨论主要发现，CCL5、CXCL5和CXCL9在钩端螺旋体病期间高度表达，这表明这些分子在疾病的免疫和发病机制中发挥着特殊的作用。对检测到的趋化因子CXCL11、CXCL9、CCL3和CCL2的相关性分析有助于阐明每种细胞因子在钩端螺旋体病中的作用。建议利用CCL5作为疾病的辅助诊断生物标志物。