Data from: Combined use of molecular markers and high resolution melting (HRM) to assess chromosome dosage in potato hybrids

In plants, the most widely used cytological techniques to assess parental
genome contributions are based on in situ hybridization (FISH and GISH),
but they are time-consuming and need specific expertise and equipment.
Recent advances in genomics and molecular biology have made PCR-based
markers a straightforward, affordable technique for chromosome typing.
Here, we describe the development of a molecular assay that uses
single-copy conserved ortholog set II (COSII)-based single nucleotide
polymorphisms (SNPs) and the high-resolution melting (HRM) technique to
assess the chromosome dosage of interspecific hybrids between a Solanum
phureja–S. tuberosum diploid (2n = 2x = 24) hybrid and its wild relative
S. commersonii. Screening and analysis of 45 COSII marker sequences
allowed S. commersonii-specific SNPs to be identified for all 12
chromosomes. Combining the HRM technique with the establishment of
synthetic DNA hybrids, SNP markers were successfully used to predict the
expected parental chromosome ratio of 5 interspecific triploid hybrids.
These results demonstrate the ability of this strategy to distinguish
diverged genomes from each other, and to estimate chromosome dosage. The
method could potentially be applied to any species as a tool to assess
paternal to maternal ratios in the framework of a breeding program or
following transformation techniques.