Cell Rejuvenation Signal Activations via Microfluidic Cell Stretch

Rejuvenation-associated research has addressed the main factors associated with the emergence of senescent cells. Although many studies in this regard have been performed to date, there is still a great need to develop novel, more efficient, and practical rejuvenation methods. The present study describes the use of microfluidics cell stretching system-induced rejuvenation (MiR) for cell rejuvenation. Microfluidics systems have a role as a solution to overcome the limitations of biology with various repertoires such as desired size, shape, and geometry. Therefore, a cellular rejuvenation study was conducted in consideration of the functional aspects of the microfluidic system. MiR downregulated senescence-associated genes. We confirmed cell recovery from senescence based on various senescence-associated parameters, including cell size, senescence-associated beta-galactosidase, reactive oxygen species, and DNA damage response. MiR modulated the rejuvenation signaling pathway by applying a physical force on the cell. Further, MiR reversed the reduction in the healing capacity of late WJ-MSCs. Thus, MiR represents an efficient new rejuvenation method in the field of regenerative medicine that can improve passage restriction, a limitation associated with the use of WJ-MSCs, and effectively increase the therapeutic efficacy of stem cells in vitro and in vivo.