QTL mapping of soybean node numbers on the main stem and meta-analysis for mining candidate genes

The soybean (<i>Glycine max</i>) node number on the main stem (NNMS) is closely related to seed yield of soybean. The aim of this study was to identify important loci affecting soybean NNMS using meta-analysis based on a reference physical map. Twenty-nine NNMS-related QTLs were mapped across 8 years with a recombination inbred line (RIL) population. Fifty-four QTLs related to NNMS of soybean identified from the database and our research were collected and each QTL was projected onto the soybean physical map. Through meta-analysis, 11 consensus soybean NNMS QTLs were obtained and located on LG D1b, LG C2, LG B1, LG F, LG L, and LG I. The map distance was from 0.02 to 4.22 Mb, the variation of original QTLs was from 2 to 4, and the mean <i>R</i>² values ranged from 6.90% to 27.40%. Furthermore, 488 candidate genes were located in these consensus QTLs, 6 of which had a relationship with NNMS. These results may help lay a foundation for fine mapping of QTLs/genes related to NNMS that can be used to help breed high-yield soybean cultivars.

大豆（Glycine max）主茎节数（node number on the main stem，以下简称NNMS）与大豆籽粒产量密切相关。本研究旨在基于参考物理图谱，通过元分析（meta-analysis）定位影响大豆NNMS的关键位点。本研究依托重组自交系（recombination inbred line，以下简称RIL）群体，历经8年试验，共定位得到29个与大豆NNMS相关的数量性状位点（quantitative trait locus，以下简称QTL）。随后，本研究从公共数据库及自身研究中收集得到54个与大豆NNMS相关的QTL，并将所有QTL投射至大豆物理图谱上。通过元分析，最终获得11个大豆NNMS一致性QTL，分别定位在连锁群（linkage group，以下简称LG）D1b、LG C2、LG B1、LG F、LG L及LG I上。该类一致性QTL的图谱区间跨度为0.02至4.22 Mb，每个一致性QTL所整合的原始QTL数量介于2至4个之间，平均表型变异解释率（R²）范围为6.90%至27.40%。此外，在上述一致性QTL区间内共注释得到488个候选基因，其中6个基因与大豆NNMS存在关联。本研究结果可为NNMS相关QTL及基因的精细定位提供理论基础，进而助力高产大豆品种的选育工作。

搜集汇总

背景与挑战

背景概述

该数据集包含大豆主茎节数（NNMS）相关的QTL定位和元分析研究，涉及29个QTL的定位和11个共识QTL的确定，以及488个候选基因的识别，旨在为高产大豆品种的育种提供基础。