Genome-wide identification of snoRNA-guided 2’-O-methylation sites [Ribometh-seq]. Homo sapiens

We combine two experimental high-throughput sequencing methods to identify new 2'-O-methylation sites in human and assign snoRNA guides to sites with previously unknown guides. Overall design: We applied RiboMeth-seq to map 2’-O-Me sites in rRNA and poly(A)-enriched RNA obtained from either the nucleus or cytoplasmic fraction of HEK293 cells. We carried out the alkaline hydrolysis for different time intervals of 8, 14 or 20 minutes. The sample titles report protocol, cell type, extraction and digestion time.