Chemometric analysis of selective polyphenolic groups in <i>Asparagus racemosus</i> (Shatavar) root extracts by traditional and supercritical fluid (CO₂) based extractions

Asparagus racemosus root extracts were prepared by supercritical fluid (CO2), soxhlet, and maceration-based methods also with various pretreatments. Thereafter, these root extracts were analyzed by High-Performance Liquid Chromatography, along with the chemometric study of the disparate phenolic groups. Among these, supercritical fluid (CO2) based extract has a larger number of polar compounds and the antioxidant activity (98.54 ± 0.22 µM Trolox equivalent mg−1). It also has the best cell viability (94.37 ± 1.12%) and insulin release (0.82 ± 1.12 ng mL−1) on β-pancreatic RINm-5F cells whereas, the best extractive yield (75.80 ± 3.44% w/w) was observed for pretreated aqueous soxhlet-based extract.