Extracellular ectonucleotidases are differentially regulated in murine tissues and human polymorphonuclear leukocytes during sepsis and inflammation

In the present studies we identified NTPDase8 a top downregulated gene in the liver of mice submitted to the cecal ligation-induced sepsis. Immunohistochemical analysis confirmed the decrease on NTPDase8 expression at the protein level. In vitro mechanistic studies using HepG2 hepatoma cells demonstrated that IL-6 but not TNF, IL-1b, bacteria or lipopolysaccharide are able to suppress NTPDase8 gene expression. RNAseq analysis of mice liver tissue in both sham and CLP (cecal ligation and puncture) sepsis model