Mineralocorticoid Receptor Antagonists and Glucocorticoids Differentially Affect Skeletal Muscle Inflammation and Pathology in Muscular Dystrophy

We report the RNA Sequencing of myleoid cells isolated from 4.5-week-old mdx mice that were administered spironolactone, prednisolone, or vehicle for 7 days. We find that both spironolactone and prednisolone downregulated expression of genes involved in inflammation and fibrosis, while prednisolone further perturbed cell cycle genes. Single cell suspensions were prepared from quadriceps from 4.5-week-old mdx mice treated with spironolactone, prednisolone, or vehicle for 7 days. Cells from 3 pairs of mdx quadriceps were pooled for each biological replicate. Myeloid cells were isolated and RNA was extracted from myeloid cells.