Sequencing of a saturated Salmonella Typhimurium TN-seq library. Construction of a tool to study the role of Salmonella Typhimurium genes: a transposon-sequencing (Tn-seq) library

Here, we present the construction and the characterization of saturated library in Salmonella Typhimurium ATCC 14028 virulent strain, which is commonly used in research laboratories. This library was obtained using the Himar1 transposon that randomly inserts into TA dinucleotides sites onto the genome. Transposon mutant library was sequenced with an Illumina NextSeq 500. After read mapping, further analyses were carried out with the TRANSIT software. These analyses confirmed saturation of the transposon mutant library both on chromosome and virulence plasmid. This allowed us to determine the essential genes and those providing advantage or disadvantage for bacterial growth.