Transcriptome analysis of bacterial blight susceptible and resistant rice lines

Rice-Xanthomonas oryzae is an economically important pathosystem owing to the loss caused by bacterial blight disease. Understanding the moleulcar dynbamics that occur during rice-Xoo interaction is crucial for understanding disaease susceptible and resistance mechanisms. SM210, harbors the resistance alleles xa5, xa13, and Xa21 and is resistant against Xoo. Transcriptome analysis of Xoo-treated. SM210 and SM, its parent variety, indicated different pathways that are altered in these varieties after Xoo treatment. Xoo strain BXO43 was cultured overnight on peptone-sucrose (PS) media. 60-day-old rice plant leaves were clip-inoculated with scissors dipped in water or Xoo (O.D. = 1.0) (Kauffman, 1973). After 24hr a 2mm leaf piece from the site of clip-inoculation was collected and frozen immediately into liquid nitrogen. 15 such leaf pieces were collected for each treatment in each set.