Gene expression profiling of cultured endometrium from women with different outcomes following in vitro fertilisation (CBX64). unidentified

The aim of this study was to differentiate the regulation of genes in the endometrium of patients with recurrent implantation failure (IF) versus those who became pregnant after IVF treatment. Moreover, the effect of embryo-derived factors on endometrial transcriptional activity was studied. Women with known IVF outcome (IF, M=Miscarriage, OP=ongoing pregnancy) and undergoing hysteroscopy with endometrial biopsy were enrolled. Biopsies were taken during the midluteal phase. After culture in presence of embryo-conditioned IVF media, total RNA was extracted and submitted to reverse transcription, target cDNA synthesis, biotin labelling, fragmentation, and hybridisation using the Affymetrix Human Genome U133A 2.0 Chip.