Raw data of transcriptome sequencing of Lactobacillus plantarum

Lactobacillus plantarum is a versatile probiotic and industrial microorganism widely used in fermented food production. However, ethanol stress during fermentation suppresses its growth and metabolic function, limiting application efficiency in ethanol-containing scenarios. This study aimed to elucidate the molecular mechanisms underlying ethanol tolerance in L. plantarum F4 via transcriptome sequencing.Experimental design: L. plantarum F4 cultures were divided into experimental (8% ethanol treatment for 24/48 h) and control (equal volume of PBS) groups, with 3 independent biological replicates per group. Cells were harvested by centrifugation, flash-frozen in liquid nitrogen, and transported on dry ice to OE Biotech Co., Ltd. (Shanghai, China) for sequencing.Sequencing and bioinformatics: Total RNA extraction, strand-specific library construction, and paired-end sequencing (Illumina platform) were performed by the company. Raw data were quality-controlled with Trimmomatic to obtain clean reads, which were aligned to the L. plantarum reference genome using Rockhopper2. Differential expression analysis was conducted via DESeq2 (p < 0.05, |log2FC| > 2), and PCA verified group separation. GO enrichment and KEGG pathway analysis of differentially expressed genes were performed using a hypergeometric test.This study is expected to identify key genes and regulatory pathways involved in L. plantarum F4, providing theoretical support for genetic modification of ethanol-tolerant strains and optimization of industrial fermentation processes.