Effect of silencing of POc04478 gene after adding 5 µM of copper ions in Penicillium oxalicum strain ?PoxKu70 at the transcriptional level.

In order to obtain genetically engineering strain of Penicillium oxalicum with high PPDE production,we silenced POc04478 gene after adding 5 µM of copper ions in Penicillium oxalicum strain ?PoxKu70 ,and we want to investigating the function of silencing of POc04478 gene after adding 5 µM of copper ions at transcriptional level. Overall design: We then performed gene expression profiling analysis using data obtained from RNA-seq of at 24 h after tansfer to the enzyme production medium with Avicel (the only carbon source).