RADseq of Haemonchus contortus

This work is the first genome-wide study in a parasitic nematode to provide direct evidence of the isotype-1 beta-tubulin locus being the single predominant benzimidazole resistance locus and provides an experimental framework for future population genomic studies on anthelmintic resistance. ddRADseq libraries were made for 80 samples, 20 per population, and sequenced with 2x75 Illumina NextSeq500. Two populations were sampled from closed sheep flocks that had an approximately 20-year history of frequent treatment exclusively with benzimidazole drugs, and two populations with a history of little or no drug treatment.