Impact of PRRX1 knockdown on the transcriptome of normal human lung fibroblasts (NHLF) in the presence or the absence of TGF-β

To assess the role of PRRX1 in TGF-β-induced lung fibroblast activation, we transfected in human NHLF with PRRX1 or control siRNAs and then exposed these cells to TGF- β (1 ng/mL). RNA samples were harvested 48 hours after TGF-β exposition. Two independent experiments were carried out. 2 independent experiments performed in a one color design, corresponding to 8 conditions (siControl#1, siControl#1+TGF-β, siControl#2, siControl#2+TGF-β, PRRX1siRNA#1, PRRX1siRNA#1 +TGF-β, PRRX1 siRNA#2, PRRX1siRNA#2 +TGF-β) for a total of 16 samples.