Ip, wallace (2010) CIL:6256, Oryctolagus cuniculus, skeletal muscle cell. CIL. Dataset

Rabbit psoas skeletal muscle fibers were blended in the presence of 5 mM MgATP to dissociate thick and thin filaments. The medium was then gently replaced with 0.3M KCl to dissociate thick filaments. Dissociated filaments were adsorbed onto mica flakes (Heuser JE, J Mol Biol. 1983 Sep 5;169(1):155-95), quick-frozen by contact with a liquid helium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 11 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev.

Images were recorded on Kodak 4489 film at 100,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner.

Stereo pair with CIL:6273.

在5 mM MgATP的存在下，将兔子腰大肌的肌纤维进行混合处理，以解离粗细肌丝。随后，以0.3M KCl轻轻更换介质，以进一步解离粗肌丝。解离后的肌丝被吸附于云母片上（Heuser JE, J Mol Biol. 1983 Sep 5;169(1):155-95），并通过接触液氦冷却的铜块在Heuser型冷冻压机上迅速冷冻，随后在Balzers 400冷冻断裂机上进行冷冻蚀刻。样品随后在11度角下以铂碳为基底进行旋转复制，并在操作于100 keV的JEOL 100CX2透射电子显微镜下进行观察。图像以100,000倍放大率记录在柯达4489胶片上。在+5度和-5度角下拍摄了立体对图像。胶片使用尼康Coolscan 9000ED扫描仪进行扫描，像素间距为20微米。与CIL:6273的立体对图像相对应。