Supplementary datasets S1 to S6 associated with FOXL2 transcription factor dynamic function during ovarian development

The transcription factor FOXL2 is required in ovarian somatic cells for female fertility. Differential timing of Foxl2 deletion, in embryonic versus adult mouse ovary, leads to distinctive outcomes, suggesting different roles across development. Here, we comprehensively investigated FOXL2’s role through a multi-omics approach to characterize gene expression dynamics and chromatin accessibility changes, coupled with genome-wide identification of FOXL2 targets and on-chromatin interacting partners in somatic cells across ovarian development. We found that FOXL2 regulates more targets postnatally, through interaction with factors regulating primordial follicle formation and steroidogenesis. Deletion of one interactor, ubiquitin-specific protease 7 (Usp7), results in impairment of somatic cell differentiation, germ cell nest breakdown, and ovarian development, leading to sterility. Our datasets constitute a comprehensive resource for exploration of the molecular mechanisms of ovarian development and causes of female infertility.

转录因子FOXL2对于卵巢体细胞的女性生育至关重要。在胚胎期与成年小鼠卵巢中，Foxl2基因敲除的时间差异导致截然不同的结果，这表明其在发育过程中的角色各异。本研究通过多组学方法全面调查了FOXL2的功能，以表征基因表达动态和染色质可及性的变化，并结合全基因组分析FOXL2在卵巢发育过程中体细胞中的靶点和染色质相互作用伙伴。我们发现，FOXL2在出生后通过调节原始卵泡形成和类固醇生成的调控因子，调节了更多的靶点。其中一个相互作用因子泛素特异性蛋白酶7（Usp7）的缺失会导致体细胞分化受损、生殖细胞巢破坏以及卵巢发育障碍，最终导致不育。我们的数据集构成了探索卵巢发育分子机制和女性不孕原因的全面资源。