Small Extracellular Vesicles (sEVs) from Human Umbilical Cord-Derived Mesenchymal Stem Cells: Spray-Drying Preservation and Therapeutic Potential in Necroptosis-Mediated Pulmonary Fibrosis Cell Models

This thesis contributes to developing an alternative approach for storing small extracellular vesicles (sEVs) derived from stem cells using a spray-drying method. Additionally, it explores the therapeutic potential of sEVs in pulmonary fibrosis (PF) by targeting the necroptosis pathway, a type of cell death. The findings suggest that spray-dried sEVs remain effective for up to 6 months in cold storage and that fresh sEVs exert anti-fibrotic and anti-necroptotic properties, thereby alleviating PF. This research provides new insights into the alternative storage forms of sEVs and their potential therapeutic applications in managing PF.