Figure S1 - The Sex Determination Gene Shows No Founder Effect in the Giant Honey Bee, Apis dorsata

Amino acid sequence alignment of region 3 of the Apis dorsata csd alleles from this research (those started with Adcsd) and those reported by Hasselmann et al. 2008 [9] (those started with Dors). A total of 54 predicted amino acid sequences were used. Alignment was done by Clustal X version 1.8 software. The alignment suggests that our sequences from DNA are truly csd alleles because they are structurally highly similar to those of [9] which were from cDNA. Both our sequences and those from Hasselmann et al. [9] contain the arginine/serine-rich (RS) domain, the hypervariable region, and the proline rich domain. Furthermore, number 53 (this study) and number 54 [9] are identical in all amino acid sequences. Others, such as 29, 30, 31 only show small differences (2 in RS domain, 1 in proline rich domain), other than the differences in the hypervariable region. (EPS)