Phenotypic analysis of LIM protein mutants in M. oryzae.

a. The diameter of colonies grown on CM plates at 25°C for 10 d. b. The conidia washed from the 15d-old CM cultures. c. Percentage of appressorium formation on GB (GelBond) surfaces incubated for 24 h or OE (onion epidermis) surfaces incubated for 48 h at 25°C. d. Percentage of invasive hyphae formation from appressoria incubated on OE for 24 h at 25°C. More than 300 spores or appressoria were counted for each strain. e. Different letters after mean values indicated significant difference at P-value of 0.05. Data were calculated from three independent experiments conducted in triplicates. Strains: Guy11, LG25 (Δrga1), GC22 (Δrga:RGA1), LD17 (Δldp1), Ku80, LP55 (Δpax1), PC20 (Δpax1:PAX1), Ku70, LR80 (Δlrg1) and RC38 (Δlrg1:LRG1).