Annotation analysis of genome, miRNA and rRNA mapping reads, for the blocked and non-blocked sRNA-seq libraries.

Shown for each sample are the number of redundant (R) and unique non-redundant (NR, unique) reads, the overall complexities (ratio of NR:R) and the proportion (Prop) of R and NR reads incident with each annotation. HT = head+thorax; AB = abdomen (subscripts f and m for female and male, respectively); AGT = male accessory glands + testes; AG = male accessory glands. For the ABm samples, for the non-blocked treatment, 89.5% of reads matched to rRNAs and only 7.6% to miRNAs. When the single oligo blocking was used, 47% of reads matched to miRNAs. For the AGT samples, 99% of reads matched to rRNAs in the non-blocked samples and 86.4% to rRNAs when the single oligo blocking was used. The proportion of miRNA annotated reads was 2.6%. For the AG tissue, the blocking increased the miRNA population from