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Expression of long non-coding RNAs during spermatogenesis

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Total RNA from purified stages of spermatogenesis were subjected to next generation RNA-seq (100bp, paired-end, strand-specific). The expression of mRNAs and lncRNAs was calculated as FPKM values. Three biological replicates of spermatogonia, pachytene and round spermatids were utilized for RNA isolation.

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