Next Generation Sequencing Facilitates Quantitative Analysis of IgG transcription in Wild Type and Bcl6-/- B cells. Mus musculus

Virus infection induces T follicular helper (TFH) and T helper 1 (TH1) cells. Although TFH cells are important in anti-viral humoral immunity, the role of TH 1 cells is still elusive. IgG2 antibodies predominate in the response to vaccination with inactivated Influenza A virus (IAV) and were responsible for protective immunity to lethal challenge with pathogenic H5N1 and pandemic H1N1 IAVs even in mice lacking TFH cells owing to B or T cell-specific ablation of the Bcl6. B cells of wild type (WT) and conditional Bcl6-/- mice were sorted and the transcription of IgG were analyzed by using Illumina MiSeq.