Microbiome of healthy and morphologically altered ileocaecal lymphnodes of slaughter pigs

Thirty-two healthy, enlarged, purulent and granulomatous lymph nodes (n=8, respectively) were selected from different slaughter pigs and isolated genomic DNA was used for pyrosequencing. 16S rRNA gene amplicons were sequenced using 454 GS FLX Titanium barcoded single-end technology to compare the microbial community of healthy and morphologically altered lymph nodes (total number of samples: 32). This study was conducted to characterize microbial community shifts associated with morphological alterations in ileocaecal lymph nodes of slaughter pigs in Austria using 16S rRNA gene-targeted-pyrosequencing. 16S rRNA genes were amplified using FLX 454 one way read fusion primers with the template specific sequence F27 - AGAGTTTGATCCTGGCTCAG and R357- CTGCTGCCTYCCGTA targeting the V1-V2 hypervariable region of the 16S rRNA gene.