Raw data for the article "Synthesis and characterization of phase-separated extracellular condensates in interactions with cells"

Biomolecular condensates formed through liquid-liquid phase separation play key roles in intracellular organization and signaling, yet their function in extracellular environments remains largely unexplored. Here, we establish a model using heparan sulfate, a key component of the extracellular matrix, to study extracellular condensate-cell interactions. We develop a methodology based on scanning probe microscopy (SPM) to quantify rheological properties of these extracellular condensates as well as their adhesion forces with cellular surfaces. Our findings and methodology open new avenues for understanding the organizational roles of condensates beyond cellular boundaries. This repository includes the data files related to this study as well as the Mathematica codes for SPM analysis (Github Link). Users are requested to cite: Naghilou et al., Chemical Engineering Journal, 164551 (2025) https://doi.org/10.1016/j.cej.2025.164551