Knockdown studies of TAPIR lncRNAs in LNCaP cells
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE134015
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To unravel the molecular function of TAPIR-1 and -2, four different specific siRNAs were used to knockdown the TAPIR-transcripts in LNCaP-cells. Gene expression changes upon knockdown of TAPIR was assessed by Agilent SurePrint G3 Human Gene Expression Arrays at 24h and 48h after treatment. Total RNA was isolated from cells with TRIzol (ambion), following the manufacturers protocol. For each siRNA type four biological replicates were prepared for two conditions: 24h and 48h after treatment.
创建时间:
2020-06-17



