<b>Single-cell RNA sequencing of rolled leaf of peanut</b>

Peanut (<i>Arachis hypogaea</i>; Leguminosae), is one of the top five global oilseed crops, holding a significant position in global oilseed production and trade. In our earlier study, we obtained a stable leaf-rolling mutant “<i>lce1</i>” using ⁶⁰Co-γ radiation. In addition to leaf-rolling, petioles, pegs, flower stalks and roots were all curving. The plant height of <i>lce1</i> were significantly lower than those of FH1 (FengHua 1, the female parent), and a smaller pod than FH1. This study intends to use the leaves of <i>lce1</i> and FH1 as materials for comparative transcriptomics analysis using scRNA-seq method, to identify the gene controlling the leaf rolling trait, so as to provide some theoretical support for peanut breeding.花生（<i>Arachis hypogaea</i>；豆科）是全球五大油料作物之一，在全球油料作物生产与贸易中占据重要地位。本团队前期研究通过⁶⁰Co-γ辐射诱变获得了稳定的卷叶突变体“lce1”，该突变体除叶片卷曲外，其叶柄、果针、花茎及根系均出现弯曲表型。lce1的株高显著低于亲本FH1（丰花1，母本），且荚果尺寸小于FH1。本研究拟以lce1与FH1的叶片为实验材料，采用单细胞RNA测序（single-cell RNA sequencing, scRNA-seq）技术开展比较转录组学分析，以期鉴定调控叶片卷曲性状的基因，为花生遗传育种提供理论支撑。

搜集汇总

背景与挑战

背景概述

该数据集通过单细胞RNA测序技术，对花生卷叶突变体'lce1'和亲本'FH1'进行对比转录组学分析，旨在鉴定控制卷叶性状的关键基因，为花生育种研究提供数据支持。数据集包含原始测序数据、细胞聚类和分析结果文件，适用于植物分子生物学领域的研究。