Determination of the influence of caNrf2 expression and/or Dextran Sulfate Sodium treatment on genome wide mRNA expression in whole colon wall or colon epithelial cells. Mus musculus

The transcription factor NF-E2-related factor 2 (Nrf2) is a major modulator of the cellular antioxidant response. To study the role of Nrf2 on mucosal inflammation we used transgenic mice expressing a constitutively active Nrf2 in epithelial cells and induced mucosal inflammation by administration of dextran sulfate sodium. Genome wide expression was studied by mRNA sequencing. Overall design: mRNA profiling of epithelial cell or colon wall samples from VilCre-caNrf2 or VilCre control mice treated with/without Dextran Sulfate Sodium, each condition was performed in triplicate