Laser Capture Microdissection-based RNAseq for Profiling Mouse and Human Sebaceous Gland Transcriptomes

We describe the use of laser capture microdissection (LCM) to isolate human and murine sebaceous glands (SGs) for transcriptomic analysis and publish this SG transcriptomic data for reference. We show that compared to whole skin RNA sequencing, LCM RNA sequencing allows for high resolution in identifying and describing SG genes at homeostasis. Lastly, we compare this LCM of sebaceous glands to published SG clusters from single cell RNA sequencing of skin, and show that we achieve greater resolution and depth with the LCM approach. Gene expression profiling analysis of laser capture microdissection derived bulk RNA-seq data from mouse and human sebaceous glands.本研究详述了利用激光捕获显微切割（laser capture microdissection, LCM）分离人和小鼠皮脂腺（sebaceous glands, SGs）以开展转录组分析的方法，并公开该皮脂腺转录组数据集以供学界参考。研究结果表明，相较于全皮肤RNA测序，激光捕获显微切割RNA测序能够在稳态条件下实现对皮脂腺基因的高分辨率鉴定与描述。最后，我们将本研究的皮脂腺激光捕获显微切割样本与已发表的皮肤单细胞RNA测序得到的皮脂腺细胞簇进行比对，证实该方法可获得更高的分辨率与测序深度。本数据集针对来源于人和小鼠皮脂腺的激光捕获显微切割样本的批量RNA测序（bulk RNA-seq）数据开展了基因表达谱分析。