Expression data from primary human healthy donor-derived CD14- cDC2s, CD14+ cDC2s and monocytes

The human dendritic cell (DC) family has recently been expanded by CD1c+CD14+CD163+ DCs, introduced as DC3. DC3 are found in tumors and peripheral blood of cancer patients but which cells can serve as their pre-cursors remain unknown. CD1c+CD14+ cells share similarities with both CD1c+ DCs (cDC2s) and CD14+ monocytes on transcriptomic and phenotypic level. To investigate whether CD14+ cDC2s are closer related to CD14- cDC2s or monocytes on transcriptomic level, we analyzed their RNA. To compare the myeloid populations on transcriptome level, RNA was isolated from FACS-sorted CD1c+CD14- DCs, CD1c+CD14+ cells and CD14+ monocytes from blood of four healthy donors and analyzed by Affymetrix microarrays.