Table_2_Cuproptosis related genes associated with Jab1 shapes tumor microenvironment and pharmacological profile in nasopharyngeal carcinoma.docx

BackgroundNasopharyngeal carcinoma (NPC) is the most common subcategory of head and neck squamous cell carcinoma (HNSCC). This study focused on the roles of cuproptosis related genes and Jab1 in the tumor microenvironment of NPC and HNSCC.MethodsDifferential expression analysis of Jab1 and cuproptosis related genes in tumor cell enriched region (PanCK-expressing) and immune cell enriched region (CD45-expressing) of NPC microenvironment were performed by packages of R software. Survival analysis was performed using the survival and survminer packages. Corrplot package was used for correlation analysis. ConsensusClusterPlus package was used for cluster clustering among different regions of NPC, and functional enrichment analysis was performed using GSVA, GSEABase, clusterProfiler, org.Hs.eg.db and enrichplot packages. The pRRophetic package was used to predict drug sensitivity in NPC and HNSCC.ResultsRelationships exist between cuproptosis related genes and Jab1 in the NPC microenvironment. The expression of cuproptosis related genes and Jab1 differed between tumor cell enriched region and immune cell enriched region. AKT inhibitor VIII, Doxorubicin, Bleomycin and Etoposide showed higher sensitivity to tumor cell than immune cell. In the high Jab1 group, higher expression of ATP7A, DBT, DLD and LIAS were associated with better prognosis of HNSCC patients. In contrast, in the low Jab1 group, higher expression of these genes is associated with worse prognosis of HNSCC patients.ConclusionsPrognostic cuproptosis related genes and Jab1 provided a basis for targeted therapy and drug development.

背景鼻咽癌（NPC）是头颈鳞状细胞癌（HNSCC）中最常见的亚型。本研究聚焦于铜死亡相关基因及Jab1在NPC及HNSCC肿瘤微环境中的作用。方法学上，通过R软件包对NPC微环境中富含肿瘤细胞区域（PanCK表达）和富含免疫细胞区域（CD45表达）中的Jab1及铜死亡相关基因进行差异表达分析。生存分析通过survival和survminer软件包执行。Corrplot软件包用于相关性分析。ConsensusClusterPlus软件包用于NPC不同区域的聚类分析，并利用GSVA、GSEABase、clusterProfiler、org.Hs.eg.db和enrichplot软件包进行功能富集分析。pRRophetic软件包用于预测NPC及HNSCC的药物敏感性。结果在NPC微环境中，铜死亡相关基因与Jab1之间存在关联。铜死亡相关基因和Jab1的表达在肿瘤细胞富含区域和免疫细胞富含区域之间存在差异。AKT抑制剂VIII、多柔比星、博来霉素和依托泊苷对肿瘤细胞的敏感性高于对免疫细胞的敏感性。在高Jab1组中，ATP7A、DBT、DLD和LIAS的高表达与HNSCC患者的良好预后相关。相反，在低Jab1组中，这些基因的高表达与HNSCC患者的较差预后相关。结论铜死亡相关基因及Jab1的预后价值为靶向治疗及药物研发提供了理论依据。