HaeIII-digested terminal restriction fragments of 16S rRNA genes, which correlated with the sediment chemical parameters (Figure 3B) of the Baltic Sea and their identification.

T-RF = terminal restriction fragment, bp = base pairs.aExpected T-RFs based on virtual digestion of partial (appr. 400−500 bp) 16S rRNA gene sequences.bObserved T-RFs (27−700 bp) produced by terminal restriction fragment length polymorphism analysis of (1) 16S rRNA gene clones and (2) 16S rRNA genes of sediment samples.cShift of 10 bp between expected and observed T-RFs was due to conditions in some of the capillary gel electrophoresis runs of digested 16S rRNA gene clones. Therefore, assignments of all T-RFs with observed lengths of 27−31 bp were used to identify T-RF 30 bp in Figure 3B, derived from the sediment samples.dBacteroidetes incertae sedis.