Pitfalls in Single Clone CRISPR-Cas9 Mutagenesis to Fine-map Regulatory Intervals

We report gene expression of four and three single-cell derived clones from HL60 and HL60/S4 cell lines, respectively, and the gene expression of bulk parental lines. We also report gene expression of control and mnocyte-derived HL60 (n=2, n=2, respectively) and HL60/S4 (n=2, n=2, respectively) single-cell clones, and control and neutrophil-derived HL60 (n=4, n=4, respectively) and HL60/S4 (n=4, n=4, respectively) single-cell clones. Overall design: Single-cell clones were derived from HL60 and HL60/S4 cell lines. The transcriptome of parental clones and bulk cells was profiled by RNA-Seq. Those single-cell derived clones were then differentiated into neutrophils and monocytes. The gene expression of vehicle controls and differentiated clones was profiled by RNA-Sequencing. Each clone with the same type of treatment or control has two replicates.