Thermodynamic parameters of the interaction between PSD-95-PDZ3 and various ligands determined by ITCa.

aExamples of the ITC experiments are shown in Figures 3 and S1. The variability in the experimental values was estimated to be approximately 1% for the number of binding sites, 5% for the binding enthalpy, and 10% for the binding affinity [29].bThe experimental conditions were 50 mM potassium phosphate (pH 7.5) at 25°C.cThe experimental conditions were 20 mM MES (pH 6.0) and 10 mM NaCl, at 25°C [19].dThe experimental conditions were 50 mM potassium phosphate (pH 7.5) at 25°C [28].eThe experimental conditions were 20 mM sodium phosphate (pH 6.8), 50 mM NaCl, and 1 mM EDTA, at 25°C [11].