The second subunit of DNA-polymerase delta is required for genomic stability and epigenetic regulation
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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DNA polymerase δ plays crucial roles in DNA repair and replication, and maintaining genomic stability. However, the function of POLD2, the second small subunit of DNA polymerase δ, has not been characterized yet in Arabidopsis. During a genetic screen for release of TGS, we identified a mutation in POLD2. Whole-genome bisulfite sequencing indicated that POLD2 is not involved in the regulation of DNA methylation. POLD2 genetically interacts with ATR and DNA polymerase α. The pold2-1 mutant exhibits genomic instability with a high frequency of homologous recombination (HR). It also exhibits hypersensitivity to DNA-damaging reagents, and short telomere length. Whole-genome ChIP-seq and RNA-seq analyses suggest that pold2-1 changes H3K27me3 and H3K4me3 modifications, and these changes are correlated with the gene expression levels. Our study suggest that POLD2 is required for maintaining genome integrity, and properly establishing the epigenetic markers during DNA replication to modulate gene expression.
提供机构：
China Agricultural University
创建时间：
2022-02-20
