Plant-associated-rhizomicrobiome Metagenome

In this work, root, rhizosphere and bulk soil samples were collected from plant-dominated ecosystems in six countries in three continents (the Americas, Africa and Asia) with different climatic zones. These included natural saline sites from the Senegal River, forest fallow sites in RD Congo, 11 and 19 year old reclaimed mining sites in South Africa, mining sites in Canada, mangrove sites in Kenya and desert sites in Kuwait. We analyzed and compared the total fungal and arbuscular mycorrhizal communities of these boreal, temperate and tropical ecosystems using Illumina Miseq of the internal transcribed spacer (ITS2) subregions and the nuclear 18S ribosomal small subunit (SSU) rRNA gene.