Table_3_Genome-Wide Identification of MATE Gene Family in Potato (Solanum tuberosum L.) and Expression Analysis in Heavy Metal Stress.XLSX

A genome-wide identification and expression analysis of multidrug and toxic compound extrusion (MATE) gene family in potato was carried out to explore the response of MATE proteins to heavy meta stress. In this study, we identified 64 MATE genes from potato genome, which are located on 12 chromosomes, and are divided into I–IV subfamilies based on phylogenetic analysis. According to their order of appearance on the chromosomes, they were named from StMATE1–64. Subcellular location prediction showed that 98% of them are located on the plasma membrane as transporters. Synteny analysis showed that five pairs of collinearity gene pairs belonged to members of subfamily I and subfamily II had two pairs indicating that the duplication is of great significance to the evolution of genes in subfamilies I and II. Gene exon–intron structures and motif composition are more similar in the same subfamily. Every StMATE gene contained at least one cis-acting element associated with regulation of hormone transport. The relative expression levels of eight StMATE genes were significantly upregulated under Cu2+ stress compared with the non-stress condition (0 h). After Cd2+ stress for 24 h, the expression levels of StMATE33 in leaf tissue were significantly increased, indicating its crucial role in the process of Cd2+ stress. Additionally, StMATE18/60/40/33/5 were significantly induced by Cu2+ stress, while StMATE59 (II) was significantly induced by Ni2+ stress. Our study initially explores the biological functions of StMATE genes in the regulation of heavy metal stress, further providing a theoretical basis for studying the subsequent molecular mechanisms in detail.

本项研究对马铃薯基因组中多药及毒性化合物外排（MATE）基因家族进行了全基因组鉴定及表达分析，旨在探究MATE蛋白对重金属胁迫的响应机制。研究从中鉴定出位于12条染色体上的64个MATE基因，根据系统发育分析结果，这些基因被划分为I至IV亚家族，并按照其在染色体上的出现顺序命名为StMATE1至64。亚细胞定位预测显示，其中98%的基因定位于质膜上，作为转运蛋白。同源分析揭示了五对连锁基因对，分别属于亚家族I和亚家族II，且亚家族II具有两对，表明这种基因重复对于亚家族I和II的基因进化具有重要意义。在同一亚家族内，基因外显子-内含子结构和基序组成更为相似。每个StMATE基因均含有至少一个与激素运输调控相关的顺式作用元件。在Cu2+胁迫条件下，与未胁迫条件（0小时）相比，八种StMATE基因的相对表达水平显著上调。在Cd2+胁迫24小时后，叶片组织中StMATE33的表达水平显著升高，表明其在Cd2+胁迫过程中的关键作用。此外，StMATE18、60、40、33和5在Cu2+胁迫下显著诱导表达，而StMATE59（II）在Ni2+胁迫下显著诱导表达。本研究首次探讨了StMATE基因在重金属胁迫调控中的生物学功能，为后续详细研究相关分子机制提供了理论依据。