CLas qPCR analysis of <i>D</i>. <i>citri</i> samples.
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CLas copy number in D. citri samples (three replicates) was estimated using qPCR targeting the 16s rRNA gene. Ct values from biological samples were compared to a dilution series of a synthetic plasmid corresponding to the CLas 16s rRNA target gene. Natural logarithm of estimated CLas copy number per insect equivalent is shown on the Y-axis. All reactions were performed in triplicate (mean plus standard deviation). No signal was observed within 40 cycles for CLas(-) samples.
创建时间：
2015-11-18
