Comparison of gene expression profilings between parental A549, OSK-A549-Colony and OSK-A549-SN. Homo sapiens

In the present study, we successfully generated lung cancer stem cell (CSC)-like cells by introducing a small set of transcription factors into a lung cancer cell line (OSK-A549-Colony). In addition to properties that are conventionally referred to as CSC properties, the lung induced CSCs exhibited the ability to form lung cancer-like tissues in vitro with vascular cells and mesenchymal stem cells, which showed structures and immunohistological patterns that were similar to human lung cancer tissues. Overall design: We extracted total RNA from 3 lung cancer cell lines (parental A549), 3 induced lung cancer stem like cells (OSK-A549-Colony) and 3 differentiated non lung cancer stem like cells (OSK-A549-SN) and hybridized them to Agilent gene expression microarrays.