Effect of influenza infection on genome structure in human primary cells [Hi-C]

Hi-C was used to profile changes in the genome structure of human primary cells at multiple time points in response to infection with active and UV-inactivated H5N1 influenza virus. Human tracheobronchial epithelial cells (HTBE) and monocyte-derived macrophages (MDM) were used. The Influenza A/Vietnam/1203/04 (H5N1) HALo mutant virus is an attenuated H5N1 virus generated from wild-type Influenza A/Vietnam/1203/04 (H5N1) virus as described in Steel, J., et al. J Virol. 2009 Feb; 83(4):1742-53. Human tracheobronchial epithelial cells and monocyte-derived macrophages, obtained from multiple donors, were infected with active and UV-inactivated H5N1 influenza. Samples were collected in duplicate at 6, 12, and 18 hours post-infection for Hi-C analysis, along with time-matched uninfected controls.