Detection of r<i>Ts</i>-Pt activity in various conditions using SDS-PAGE zymography.
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(A) SDS-PAGE zymography analysis of rTs-Pt in the presence of various metal ions. The gel was stained with ethidium bromide. The numbers 1, 2, 3, 4, 5, 6, and 7 indicate the presence of 0 mM, 5 mM, 10 mM, 20 mM, 30 mM, 40 mM, and 50 mM metal ions, respectively. (B) SDS-PAGE zymography analysis of rTs-Pt in the presence of 1 mM EDTA. Lane 1, normal; Lane 2, EDTA. (C) SDS-PAGE zymography analysis of rTs-Pt in the presence of ATA. The gel was stained with ethidium bromide. The numbers 1, 2, 3, 4, 5, and 6 indicate the presence of 0 µM, 1 µM, 5 µM, 15 µM, 20 µM, and 25 µM ATA, respectively. (D) SDS-PAGE zymography analysis of rTs-Pt in different pH conditions. The gel was stained with ethidium bromide. The numbers 1, 2, 3, 4, 5, and 6 indicate pH 3, pH 4, pH 5, pH 6, pH 7, and pH 8, respectively. (E) SDS-PAGE zymography analysis of rTs-Pt in different temperatures. The gel was stained with ethidium bromide. The numbers 1, 2, 3, 4, 5, 6, and 7 indicate 16°C, 20°C, 25°C, 30°C, 37°C, 42°C, and 50°C, respectively.
