Gene expression alterations with Nkapl knockout or NKAPL frameshift mutation (M349fs)

This study aims to explore the transcriptomic changes in Nkapl-KO, Sox30-KO and Nkapl TGdel/TGdel mouse testes comparing with their wild-type testes. Fresh testes were collected from 21d wild-type, Nkapl-KO, Sox30-KO and Nkapl TGdel/TGdel mice, respectively. For each group, we have biological triplicates. Total RNA was extracted using TRIzol reagent. Strand-specific libraries were prepared using the TruSeq Stranded Total RNA Sample Preparation kit (Illumina) according to the manufacturer's instructions.本研究旨在探究Nkapl敲除（Nkapl-KO）、Sox30敲除（Sox30-KO）及Nkapl TGdel/TGdel小鼠睾丸与野生型小鼠睾丸的转录组变化。研究分别从21日龄野生型、Nkapl-KO、Sox30-KO及Nkapl TGdel/TGdel小鼠体内采集新鲜睾丸组织，每组设置3个生物学重复。总RNA采用TRIzol试剂提取；链特异性文库的构建依照制造商说明书，使用TruSeq Stranded Total RNA样本制备试剂盒（Illumina）完成。