Global gene expression analysis of vascular progenitors differentiated from human embryonic stem cells or induced pluripotent stem cells and their corresponding donor starting cells. Homo sapiens

Global gene expression analysis of CD31+ / CD146 +/- vascular progenitors (VP) derived from (a) human embryonic stem cells (VP-hESC), (b) adult fibroblast derived induced pluripotent stem cells (VP-AdF-iPSC), and (c) stromal primed cord blood CD34+ myeloid progenitor derived iPSC (VP-sp-CB-iPSC), (d) corresponding iPSC, and (e) corresponding starting fibroblasts and myeloid progenitors. Overall design: Total RNA was harvested from (a) adult fibroblasts, (b) human myeloid progenitors: Non-nucloefected CD34+ CB cells that were expanded with growth factors from Day -3, and co-cultured with bone marrow stromal cells (BMSC) from Day 0 until Day+3. CD45+ myeloid progenitors were FACS-purified on Day +3 (c) FACS-purified CD31+ CD146 +/- vascular progenitors (VP) that were differentiated from human embryonic stem cells (hESC), (d) VP differentiated from low passage stromal-primed 7-factor episomal iPSC derived from growth-factor activated cord blood myeloid progenitors, and (e) VP differentiated from 4 factor viral construct iPSC derived from adult fibroblasts. Illumina HumanHT-12 V4.0 expression beadchips were used for all analyses in this series. Three independent samples of each condition were each run on individual microarrays.