Transcriptional effects of catalytic mTOR inhibiton among LPS-stimulated primary human monocytes

To better understand the role of mTOR in regulation of the monocyte response to LPS, we obtained mRNA-seq profiles of primary human monocytes stimulated ex vivo through TLR4, with and without pretreatment with a catalytic mTOR inhibitor (mTORi) Overall design: We obtained primary human monocytes from six independent donors and stimulated with LPS (1 ng/mL) or vehicle for 6h following pretreatment with an mTORi (AZD2014 at 5µM) or DMSO for 6h ex vivo, prior to extraction of RNA and library prep.