five

Fluctuating starvation conditions modify host-symbiont relationship between a leaf beetle and its newly identified gregarine species|生态学数据集|寄生虫学数据集

收藏
Mendeley Data2024-04-13 更新2024-06-28 收录
生态学
寄生虫学
下载链接:
https://datadryad.org/stash/dataset/doi:10.5061/dryad.fxpnvx0tk
下载链接
链接失效反馈
资源简介:
Study Organism and Rearing The leaf beetle P. cochleariae was reared for several generations at 20 °C, 16 h: 8 h light:dark, 70% r.h. in a climate cabinet and once a year crossed with field-collected beetles (51°51′21″ N, 8°41′37″ E). Groups of about 150 individuals were kept together in plastic rearing boxes (20 x 20 x 6.5 cm) with gauze lids. As food and for oviposition, leaves of Chinese cabbage (Brassica rapa spp. pekinensis) were provided. The plants were grown in a greenhouse (20˚C, 16 h: 8 h light:dark, 70% r.h.) and only 7-10 weeks old non-flowering plants were used. Experimental Set-up to Test Effects of Gregarine and Fluctuating Starvation on Life-History Traits of Beetles To test consequences of a gregarine on its host under different environmental conditions, a full-factorial set-up was used with gregarines (G-, G+) and fluctuating starvation (S-, S+) as treatments, resulting in four treatment groups (G-S-, G-S+, G+S-, G+S+) with 67 – 70 replicates per group. For the experiment, cabbage leaves were offered for 24 h to the beetles in one rearing box. Then, eggs were randomly collected from these leaves. For gregarine infection we followed a protocol developed previously in our group (Wolz et al., 2022). Each egg was carefully cleaned from faecal residues and female secretions with a moist brush. Eggs were then randomly distributed to one of two gregarine treatment groups (uninfected and infected), kept each in a rearing box with fresh cabbage leaves. Shortly before larval hatching (after 6 d), individuals of the uninfected (G-) treatment group received cabbage leaves that had been mechanically damaged by knife cuttings (to provide leaves of comparable quality between treatment groups) and left for 48 h in a rearing box without any insects. Individuals of the gregarine-infected treatment (G+) group received cabbage leaves, which had served as food in rearing boxes with beetles for 48 h and were contaminated with faeces from gregarine-infected conspecifics. Faecal residues contain oocysts with infectious sporozoites, which are ingested by the larvae and cause gregarine infection. Larvae of the two gregarine treatment groups received the respective food, which was replaced every 24 h, for three days. On the fourth day after larval hatching, all individuals received untreated cabbage leaves, which were replaced every 48 h, until the end of the experiment (except during starvation periods). Within both gregarine treatment groups (G- and G+), larvae were subdivided into groups of five in Petri dishes (9 cm diameter) lined with filter paper. Half of the individuals of each of the gregarine treatment groups were fed ad libitum and assigned to the non-starvation treatment group (S-), the other half were starved three times (starvation group, S+), each time for a period of 24 h (d4, d7 and d11 after larval hatching), as similarly performed in an earlier study with sawfly larvae (Paul et al., 2019). In the field, larvae may experience repeated bouts of starvation when their host plants are over-exploited by high population densities. To prevent any potential cannibalism and to keep up humidity, small moistened paper balls were added to the Petri dishes during the starvation periods, providing hiding places. However, cannibalism never occurred in this or former experiments with this species. To investigate the impact of this fluctuating starvation treatment and larval body mass on the number of gregarines, one larva was taken from each Petri dish on d13 after larval hatching (n = 13 per group; for gregarine counting see below). Remaining larvae that pupated were placed individually into Petri dishes (5 cm diameter) lined with filter paper. The day of adult eclosion was noted to determine the development time from larval hatch to reaching adulthood in dependence of the treatments. Two days after adult hatching, the beetles were sexed, weighed (micro balance, ME36S, Sartorius AG, Göttingen, Germany) and adult biomass used as further life-history parameter. Pairs of one male and one female were set up for mating within each treatment group (mating pairs: G-S-: n = 31, G-S+: n = 20, G+S-: n = 20, G+S+: n = 10). The pairs remained together until the seventh day after adult hatching, after which the males were removed. The females were weighed again and the number of eggs laid was counted for each female for four consecutive days (from day 7-10 after adult hatching) as measure of fecundity. From larval hatching until the seventh day of adulthood, the number of individuals that had died were monitored daily to calculate the probability of survival. As the sex of larvae cannot be determined, these data were not separated by sex. The adults can usually live up to three months under laboratory conditions. Counting Total Number of Gregarines in Hosts of the Different Treatments Larvae taken for gregarine counting (see above) were weighed and frozen at -20 °C (14 replicates per treatment group). To determine the total number of gregarines (only trophozoite stage) in the gut, the larvae were dissected, and their guts spread in sodium phosphate buffer (0.1 M, pH = 7.2) on microscope slides. The trophozoites were counted at 200 to 400 times magnification (ZEISS Axiophot). Statistical Analyses The statistical processing and visualisation of the data were performed with R (version 3.6.3, R Core Team, 2020) in RStudio (version 1.2.5033, RStudio Team, 2019). Model residuals were tested for normality and variance homogeneity and stepwise backwards deletion of non-significant interaction terms and predictors (F test or Chisq test) was computed to obtain the minimum adequate models (package: MASS; Venables and Ripley, 2002). In the results section, only the statistical values of the predictors that remained in the final models are reported. The effects of gregarine treatment, starvation treatment and their interaction on development time and number of eggs laid by females were tested using generalised linear models [GLMs: poisson distribution and identity link function (development time) or link log function (egg number)]. Treatment effects on the body mass of adult beetles were analysed separately for males and females by linear models (LMs: Gaussian distribution, identity link function). The treatment effects on survival data were analysed by a stratified cox model to control for proportional hazard assumption (package: survival, Therneau, 2020). The effects of starvation treatment, insect body mass and their interaction on the number of gregarines were tested using a GLM (poisson distribution, identity link function).
创建时间:
2023-06-28
用户留言
有没有相关的论文或文献参考?
这个数据集是基于什么背景创建的?
数据集的作者是谁?
能帮我联系到这个数据集的作者吗?
这个数据集如何下载?
点击留言
数据主题
具身智能
数据集  4098个
机构  8个
大模型
数据集  439个
机构  10个
无人机
数据集  37个
机构  6个
指令微调
数据集  36个
机构  6个
蛋白质结构
数据集  50个
机构  8个
空间智能
数据集  21个
机构  5个
5,000+
优质数据集
54 个
任务类型
进入经典数据集
热门数据集

Materials Project 在线材料数据库

Materials Project 是一个由伯克利加州大学和劳伦斯伯克利国家实验室于 2011 年共同发起的大型开放式在线材料数据库。这个项目的目标是利用高通量第一性原理计算,为超过百万种无机材料提供全面的性能数据、结构信息和计算模拟结果,以此加速新材料的发现和创新过程。数据库中的数据不仅包括晶体结构和能量特性,还涵盖了电子结构和热力学性质等详尽信息,为研究人员提供了丰富的材料数据资源。相关论文成果为「Commentary: The Materials Project: A materials genome approach to accelerating materials innovation」。

超神经 收录

MedDialog

MedDialog数据集(中文)包含了医生和患者之间的对话(中文)。它有110万个对话和400万个话语。数据还在不断增长,会有更多的对话加入。原始对话来自好大夫网。

github 收录

Traditional-Chinese-Medicine-Dataset-SFT

该数据集是一个高质量的中医数据集,主要由非网络来源的内部数据构成,包含约1GB的中医各个领域临床案例、名家典籍、医学百科、名词解释等优质内容。数据集99%为简体中文内容,质量优异,信息密度可观。数据集适用于预训练或继续预训练用途,未来将继续发布针对SFT/IFT的多轮对话和问答数据集。数据集可以独立使用,但建议先使用配套的预训练数据集对模型进行继续预训练后,再使用该数据集进行进一步的指令微调。数据集还包含一定比例的中文常识、中文多轮对话数据以及古文/文言文<->现代文翻译数据,以避免灾难性遗忘并加强模型表现。

huggingface 收录

UAVDT

UAVDT是一个用于目标检测任务的数据集。

github 收录

中国地质调查局: 全国1∶200 000区域水文地质图空间数据库

全国1∶200 000区域水文地质图空间数据库以建国后在全国范围内(本次未在香港特别行政区、澳门特别行政区和台湾省开展工作) 30个省开展的1∶200 000区域水文地质普查工作所取得的区域水文地质普查报告、综合水文地质图等地质资料为数据源,在制定的“1∶200 000区域水文地质图空间数据库图层及属性文件格式标准”的基础上,建成了一个全国性的、大型的区域水文地质学空间数据库。该数据库总共采集、处理了全国范围内1∶200 000图幅的<number>1 017</number>幅全要素综合水文地质图信息,全部数据量约50 GB。数据库涵盖了以1∶200 000国际标准图幅为管理单位的水文地质要素空间数据图层,内容包括:地理要素(交通层、水系层、行政区划层等),基础地质要素(地层分区层、断裂构造层),水文地质要素(地下水类型层、地下水富水性层、地下水迳流模数层,地下水水质层、水文地质特征层、地下水利用规划层),专题要素(综合水文地质柱状图,水文地质剖面图) 四大类近30个要素图层。空间数据库主要采用MapGIS地理信息系统格式存储,形成了目前国内覆盖范围最广、包含信息最完整的区域水文地质图空间数据库成果,是地质领域全国性最重要的基础信息资源之一。

DataCite Commons 收录