Glucose concentration levels in serums and IVD tissues of wild-type and APOE-knockout rabbits.

Glucose concentrations in serums [μmol/ml] as well as in NP and AF tissues [nmol/mg] were determined using the glucose-oxidase assays. Compared to that in serums of wild-type rabbits (WT-Serum) higher glucose concentration was detected in serums of APOE-knockout rabbits (KO-Serum). In contrast, lower glucose concentrations were confirmed in NP and AF tissues of APOE-knockout rabbits (KO-NP, KO-AF). In addition lower glucose concentrations were detected in NP tissues (WT-NP, KO-NP) than in AF tissues (WT-AF, KO-AF) of the same rabbit group. Three independent glucose-oxidase assays were made in duplicate for each sample and significance was set at p