Whole-genome gene expression comparison of the chronic myeloid leukemia cell line K562 and its tyrosine kinase inhibitor resistant subclone K562-IR.. Whole-genome gene expression comparison of the chronic myeloid leukemia cell line K562 and its tyrosine kinase inhibitor resistant subclone K562-IR.

The aim of the analysis is to study the relationship between tyrosine kinase inhibitor (TKI) resistance mechanism and phenotypic plasticity in the TKI-resistant and parental chronic myeloid leukemia K562 cell lines, in the presence and absence of imatinib. Results provide insight into the molecular mechanisms underlying the acquisition of cancer cell plasticity. Overall design: 4 group of cell lines were studied in triplicate (12 samples) for the microarray analysis. K562 samples were used as reference. K562-IR w/ imatinib cells (suspension) and spindle-shape K562-IR cells (adhering under the suspension cells) were incubated continuously with 10µM imatinib. K562-IR w/o imatinib cells were centrifuged to remove imatinib, and then cultured in complete media during 4 week before collecting for the analysis. K562-IR w/ imatinib cells were collected directly using centrifugation, and spindle-shape K562-IR cells were trypsinized then collected by centrifugation because they grows as monolayer.