Sequencing of Recombinant Baculovirus Vectors - p6.9 rBEV
收藏DataCite Commons2025-11-20 更新2025-04-09 收录
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https://borealisdata.ca/citation?persistentId=doi:10.5683/SP3/FIBEX4
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2nd passage (P2) recombinant baculovirus vectors having a reporter green fluorescent protein (mAzami Green) under the control of the p6.9 promoter. The recombinant viruses were amplified in Spodoptera frugiperda (Sf9) cells at a low multiplicity of infection (MOI), ~0.1, and harvested 72 hours post infection (hpi). The baculovirus genomic DNAs (gDNAs) from the propagated P2 p6.9GFP rBEV stock or P2 p6.9GFP_sgRNA_gp64+131 rBEV stock were extracted and sequenced via shotgun sequencing or tiled-amplicon sequencing, respectively. Control and CRISPR-Cas9 targeted baculovirus genomes, upon plasmid or rBEV-based delivery of sgRNA into Sf9-Cas9 cells, were extracted and sequenced via tiled-amplicon sequencing.
提供机构：
Borealis
创建时间：
2025-01-09
